Western blotting analysis using anti-FLAG-tag antibody (Cat#52108). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLAG-tag antibody (Cat#52108, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).