Western blotting analysis using anti-HA-tag antibody (Cat#52109). Recombinant multi-tag protein (5 ng) expressed by E. coli were loaded and separated by SDS-PAGE. The blot was incubated with anti-HA-tag antibody (Cat#52109, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).