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Anti-PSMA4 Rabbit Monoclonal Antibody #5216

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Flow cytometric analysis of PSMA4 expression in HepG2 cells using anti-PSMA4 antibody (Cat#5216, 1:2,000). Green, isotype control; red, PSMA4.

Immunocytochemical staining of HepG2 cells with anti-PSMA4 antibody (Cat#5216, 1:1,000) . Nuclei were stained blue with DAPI; PSMA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PSMA4 antibody (Cat#5216). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMA4 antibody (Cat#5216, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PSMA4
货号：

5216
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-PSMA4 Rabbit Monoclonal Antibody
Aliases	PSMA4; Proteasome 20S Subunit Alpha 4; HC9; HsT17706; Proteasome (Prosome, Macropain) Subunit, Alpha Type, 4; Multicatalytic Endopeptidase Complex Subunit C9; Proteasome Subunit Alpha Type-4; Proteasome Subunit Alpha 4; Proteasome Subunit Alpha-3; Proteasome Component C9; Macropain Subunit C9; Proteasome Subunit L; Alpha-3; PSC9; Proteasome Subunit Alpha3; Proteasome Subunit HC9; Proteasome Subunit Α3; EC 3.4.25.1
Background	Gene Name: PSMA4 NCBI Gene Entry: 5685 UniProt Entry: P25789
Application Information	Molecular Weight: Predicted, 29 kDa; observed, 29 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB205 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PSMA4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:1,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PSMA4 expression in HepG2 cells using anti-PSMA4 antibody (Cat#5216, 1:2,000). Green, isotype control; red, PSMA4.
Immunocytochemical staining of HepG2 cells with anti-PSMA4 antibody (Cat#5216, 1:1,000) . Nuclei were stained blue with DAPI; PSMA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PSMA4 antibody (Cat#5216). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMA4 antibody (Cat#5216, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PSMA4
货号：

5216
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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