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Anti-RPS6KA2 Rabbit Monoclonal Antibody #5219

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Flow cytometric analysis of RPS6KA2 expression in HeLa cells using anti-RPS6KA2 antibody (Cat#5219, 1:2,000). Green, isotype control; red, RPS6KA2.
Immunocytochemical staining of Hela cells with anti-RPS6KA2 protein antibody (Cat#5219, 1:1,000) . Nuclei were stained blue with DAPI; RPS6KA2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RPS6KA2 antibody (Cat#5219). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RPS6KA2 antibody (Cat#5219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    RPS6KA2
  • 货号:
    5219
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-RPS6KA2 Rabbit Monoclonal Antibody
Aliases RPS6KA2; Ribosomal Protein S6 Kinase A2; RSK3; HU-2; MAP Kinase-Activated Protein Kinase 1c; Ribosomal Protein S6 Kinase Alpha-2; MAPK-Activated Protein Kinase 1c; Ribosomal S6 Kinase 3; EC 2.7.11.1; MAPKAPK1C; Pp90RSK3; P90RSK2; Mitogen-Activated Protein Kinase-Activated Protein Kinase 1C; Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 2; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 2; 90 KDa Ribosomal Protein S6 Kinase 2; MAPKAP Kinase 1c; S6K-Alpha-2; S6K-Alpha2; MAPKAPK-1c; S6K-Alpha; P90-RSK 2; EC 2.7.11; P90-RSK3; RSK-3; RSK
Background

Gene Name: RPS6KA2

NCBI Gene Entry: 6193

UniProt Entry: Q15349

Application Information

Molecular Weight: Predicted, 83 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB220

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human RSK3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:1,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RPS6KA2 expression in HeLa cells using anti-RPS6KA2 antibody (Cat#5219, 1:2,000). Green, isotype control; red, RPS6KA2.
  • Immunocytochemical staining of Hela cells with anti-RPS6KA2 protein antibody (Cat#5219, 1:1,000) . Nuclei were stained blue with DAPI; RPS6KA2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-RPS6KA2 antibody (Cat#5219). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RPS6KA2 antibody (Cat#5219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of RPS6KA2 expression in HeLa cells using anti-RPS6KA2 antibody (Cat#5219, 1:2,000). Green, isotype control; red, RPS6KA2.
Immunocytochemical staining of Hela cells with anti-RPS6KA2 protein antibody (Cat#5219, 1:1,000) . Nuclei were stained blue with DAPI; RPS6KA2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RPS6KA2 antibody (Cat#5219). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RPS6KA2 antibody (Cat#5219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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