Western blotting analysis using anti-PPAR Gamma antibody (Cat#52318). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPAR Gamma antibody (Cat#52318, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Tissue/cell: rat aorta tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-PPAR gamma Rabbit Polyclonal Antibody, Unconjugated 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen
A synthesized peptide derived from human PPAR Gamma
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry (IHC): 1:100-1:500
Note
This product is for research use only.
Picture
Western blotting analysis using anti-PPAR Gamma antibody (Cat#52318). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPAR Gamma antibody (Cat#52318, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Tissue/cell: rat aorta tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-PPAR gamma Rabbit Polyclonal Antibody, Unconjugated 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Western blotting analysis using anti-PPAR Gamma antibody (Cat#52318). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPAR Gamma antibody (Cat#52318, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Tissue/cell: rat aorta tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-PPAR gamma Rabbit Polyclonal Antibody, Unconjugated 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
