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Anti-HIF-1 alpha Rabbit Polyclonal Antibody #52366

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Western blotting analysis using anti-HIF-1 alpha antibody (Cat#52366). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF-1 alpha antibody (Cat#52366, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    HIF1A
  • 货号:
    52366
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-HIF-1 alpha Rabbit Polyclonal Antibody
Aliases HIF1A; Hypoxia Inducible Factor 1 Subunit Alpha; BHLHe78; PASD8; MOP1; Class E Basic Helix-Loop-Helix Protein 78; PAS Domain-Containing Protein 8; Member Of PAS Protein 1; HIF-1alpha; HIF1; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor); Basic-Helix-Loop-Helix-PAS Protein MOP1; Hypoxia-Inducible Factor 1-Alpha; HIF-1-Alpha; Hypoxia Inducible Factor 1 Alpha Subunit; Hypoxia-Inducible Factor1alpha; Member Of PAS Superfamily 1; ARNT Interacting Protein; ARNT-Interacting Protein; HIF1-ALPHA; HIF1-Alpha; BHLHE78; HIF-1A
Background

NCBI Gene Entry: 3091

UniProt Entry: Q16665

Application Information

Molecular Weight: Predicted, 93 kDa; observed, 92-130 kDa

Clonality: Rabbit polyclonal antibody

Clone ID: N/A

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human HIF-1alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,000
Immunohistochemistry (IHC): 1:100-1:300
Immunocytochemistry (IC): 1:50-1:200
Immunoprecipitation (IP): 1:200
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-HIF-1 alpha antibody (Cat#52366). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF-1 alpha antibody (Cat#52366, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-HIF-1 alpha antibody (Cat#52366). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF-1 alpha antibody (Cat#52366, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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