Anti-Phospho-IKB alpha (S32) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-IKB alpha (S32) Rabbit Monoclonal Antibody #5241

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Flow cytometric analysis of Phospho-IKB alpha (S32) expression in HepG2 cells using anti-Phospho-IKB alpha (S32) antibody (Cat#5241, 1:2,000). Green, isotype control; red, Phospho-IKB alpha (S32).

Immunocytochemical staining of HepG2 cells with Phospho-IKB alpha (S32) antibody (Cat#5241, 1:1,000). Nuclei were stained blue with DAPI; Phospho-IKB alpha (S32) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-IKB alpha (S32) antibody (Cat#5241). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-IKB alpha (S32) antibody (Cat#5241, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NFKBIA
货号：

5241
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Phospho-IKB alpha (S32) Rabbit Monoclonal Antibody
Aliases	ENFKBIA; NFKB Inhibitor Alpha; IKBA; NF-Kappa-B Inhibitor Alpha; IkappaBalpha; MAD-3; NFKBI; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha; Major Histocompatibility Complex Enhancer-Binding Protein MAD3; I-Kappa-B-Alpha; IkB-Alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B-Cells; IKAPPABALPHA 5; EDAID2; MAD3
Background	Gene Name: NFKBIA NCBI Gene Entry: 4792 UniProt Entry: P25963
Application Information	Molecular Weight: Predicted, 36 kDa; observed, 39 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1140 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-IKB alpha (S32)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Phospho-IKB alpha (S32) expression in HepG2 cells using anti-Phospho-IKB alpha (S32) antibody (Cat#5241, 1:2,000). Green, isotype control; red, Phospho-IKB alpha (S32).
Immunocytochemical staining of HepG2 cells with Phospho-IKB alpha (S32) antibody (Cat#5241, 1:1,000). Nuclei were stained blue with DAPI; Phospho-IKB alpha (S32) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-IKB alpha (S32) antibody (Cat#5241). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-IKB alpha (S32) antibody (Cat#5241, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NFKBIA
货号：

5241
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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