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Anti-POU5F Rabbit Monoclonal Antibody #52442

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Western blotting analysis using anti-POU5F antibody (Cat#52442). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-POU5F antibody (Cat#52442, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    POU5F
  • 货号:
    52442
  • 应用:
    WB, IHC, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-POU5F Rabbit Monoclonal Antibody
Aliases POU5F; POU Class 5 Homeobox; OCT3; Oct3/4; OCT-4; Oct4; OTF3; POU Domain, Class 5, Transcription Factor 1; Octamer-Binding Transcription Factor 3; Octamer-Binding Protein 3; Octamer-Binding Protein 4; MGC22487; OTF-3; Oct-3; POU-Type Homeodomain-Containing DNA-Binding Protein ; POU Domain Class 5, Transcription Factor 1; POU Domain Transcription Factor OCT4; Oct-4; OTF4; OCT4
Background

Gene Name: POU5F

NCBI Gene Entry: 5460

UniProt Entry: Q01860

Application Information

Molecular Weight: Predicted, 39 kDa; observed, 45 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human POU5F
Isotype Rabbit IgG,Kappa
Storage Buffer Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.04% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
IImmunohistochemistry (IHC): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:200-1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-POU5F antibody (Cat#52442). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-POU5F antibody (Cat#52442, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-POU5F antibody (Cat#52442). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-POU5F antibody (Cat#52442, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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