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Anti-RFC2 Rabbit Monoclonal Antibody #5248

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Flow cytometric analysis of RFC2 expression in HeLa cells using anti-RFC2 antibody (Cat#5248, 1:2,000). Green, isotype control; red, RFC2.

Immunocytochemical staining of Hela cells with anti-RFC2 protein antibody (Cat#5248, 1:1,000) . Nuclei were stained blue with DAPI; RFC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-RFC2 antibody (Cat#5248). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RFC2 antibody (Cat#5248, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

RFC2
货号：

5248
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-RFC2 Rabbit Monoclonal Antibody
Aliases	RFC2; Replication Factor C Subunit 2; RFC40; Replication Factor C (Activator 1) 2, 40kDa; Activator 1 Subunit; RF-C 40 KDa Subunit; A1 40 KDa Subunit; A1; Replication Factor C (Activator 1) 2 (40kD); Replication Factor C 40 KDa Subunit; Activator 1 40 KDa Subunit; Activator 1
Background	Gene Name: RFC2 NCBI Gene Entry: 5982 UniProt Entry: P35250
Application Information	Molecular Weight: Predicted, 39 kDa; observed, 37 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB345 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human RFC2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:1,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of RFC2 expression in HeLa cells using anti-RFC2 antibody (Cat#5248, 1:2,000). Green, isotype control; red, RFC2.
Immunocytochemical staining of Hela cells with anti-RFC2 protein antibody (Cat#5248, 1:1,000) . Nuclei were stained blue with DAPI; RFC2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-RFC2 antibody (Cat#5248). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RFC2 antibody (Cat#5248, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

RFC2
货号：

5248
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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