Western blotting analysis using anti-ZO-1 antibody (Cat#52486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZO-1 antibody (Cat#52486, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
TJP1; Tight Junction Protein 1; Tight Junction Protein ZO-1; ZO-1; Zona Occludens 1; DKFZp686M05161; MGC133289; Zonula Occludens 1 Protein; Zonula Occludens Protein 1; Zona Occludens Protein 1; ZO1
Supplied in 50mM Tris-Glycine (pH 7.4 )containing 40% glycerol, 0.15M NaCl, 0.01% sodium azide and 0.05% BSA.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Picture
Western blotting analysis using anti-ZO-1 antibody (Cat#52486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZO-1 antibody (Cat#52486, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ZO-1 antibody (Cat#52486). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ZO-1 antibody (Cat#52486, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
