Paraformaldehyde-fixed, paraffin embedded mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with WNT11 antibody (Cat#52643) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Western blotting analysis using anti-WNT11 antibody (Cat#52643). Total lysates (40 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-WNT11 antibody (Cat#52643, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human WNT11
Isotype
Rabbit IgG
Storage Buffer
Supplied in TBS (pH 7.4) containing 50% glycerol, 1% BSA and 0.02% Proclin 300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,000 Immunohistochemistry(IHC): 1:100-1:500 Immunocytochemistry (IC): 1:100-1:500
Note
This product is for research use only.
Picture
Paraformaldehyde-fixed, paraffin embedded mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with WNT11 antibody (Cat#52643) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Western blotting analysis using anti-WNT11 antibody (Cat#52643). Total lysates (40 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-WNT11 antibody (Cat#52643, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
Paraformaldehyde-fixed, paraffin embedded mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with WNT11 antibody (Cat#52643) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Western blotting analysis using anti-WNT11 antibody (Cat#52643). Total lysates (40 μg) were loaded and separated by SDS-PAGE.The blot was incubated with anti-WNT11 antibody (Cat#52643, 1:1,000) and IRDye800CW-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively.
