Immunocytochemical analysis of SIRT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of SIRT2 expression in PC12 (A), rat brain (B), mouse brain (C) whole cell lysates.
SIRT2; Sirtuin 2; SIR2L; NAD-Dependent Protein Defatty-Acylase Sirtuin-2; NAD-Dependent Protein Deacetylase Sirtuin-2; Regulatory Protein SIR2 Homolog 2; SIR2-Like Protein 2; SIR2L2; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 2 (S. Cerevisiae); Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog) 2; NAD-Dependent Deacetylase Sirtuin-2; Silent Information Regulator 2; Sir2-Related Protein Type 2; Sirtuin Type 2; EC 2.3.1.286; EC 2.3.1.-; SIR2
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
Recombinant protein of human SIRT2
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.3) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000 Immunocytochemistry (IC): 1:50-1:100
Note
This product is for research use only.
Picture
Immunocytochemical analysis of SIRT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of SIRT2 expression in PC12 (A), rat brain (B), mouse brain (C) whole cell lysates.
Immunocytochemical analysis of SIRT2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Western blotting analysis of SIRT2 expression in PC12 (A), rat brain (B), mouse brain (C) whole cell lysates.
