Western blotting analysis using anti-Complement C3 antibody (Cat#52831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Complement C3 antibody (Cat#52831, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
A synthesized peptide derived from human Complement C3
Isotype
Rabbit IgG
Storage Buffer
Supplied in 0.01M TBS (pH7.4) containing 50% glycerol, 1% BSA and 0.02% Proclin300.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Picture
Western blotting analysis using anti-Complement C3 antibody (Cat#52831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Complement C3 antibody (Cat#52831, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Complement C3 antibody (Cat#52831). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Complement C3 antibody (Cat#52831, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).