Western blotting analysis using anti-RSBN1 antibody (Cat#52896). Total lysates (20 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-RSBN1 antibody (Cat#52896, 1:2,000) and Peroxidase conjugated goat anti-rabbit IgG secondary antibody respectively.
Immunohistochemical analysis of paraffin-embedded human kidney. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with RSBN1 antibody (Cat#52896, 1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Applications Tested: Western blotting (WB), immunohistochemistry-Paraffin (IHC-P), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human RSBN1
Isotype
Rabbit IgG
Storage Buffer
supplied in PBS containing 50% glycerol, and 0.02% sodium azide
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB):1:500-1:2,000 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:500 Flow Cytometry (FCM): 1:10-1:50
Note
This product is for research use only.
Picture
Western blotting analysis using anti-RSBN1 antibody (Cat#52896). Total lysates (20 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-RSBN1 antibody (Cat#52896, 1:2,000) and Peroxidase conjugated goat anti-rabbit IgG secondary antibody respectively.
Immunohistochemical analysis of paraffin-embedded human kidney. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with RSBN1 antibody (Cat#52896, 1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Western blotting analysis using anti-RSBN1 antibody (Cat#52896). Total lysates (20 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-RSBN1 antibody (Cat#52896, 1:2,000) and Peroxidase conjugated goat anti-rabbit IgG secondary antibody respectively.
Immunohistochemical analysis of paraffin-embedded human kidney. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with RSBN1 antibody (Cat#52896, 1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
