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Anti-Adenylate Kinase 1 Mouse Monoclonal Antibody #5329

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Flow cytometric analysis of Adenylate kinase 1 expression in HepG2 cells using anti-Adenylate kinase 1 antibody (Cat#5329, 1:2,000). Green, isotype control; red,Adenylate kinase 1 .

Immunocytochemical staining of HepG2 cells with anti-Adenylate kinase 1 antibody (Cat#5329, 1:1,000). Nuclei were stained blue with DAPI; Adenylate kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-adenylate kinase 1 antibody (Cat#5329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adenylate kinase 1 antibody (Cat#5329, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

AK1
货号：

5329
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Adenylate Kinase 1 Mouse Monoclonal Antibody
Aliases	AK1; Adenylate Kinase 1; Adenylate Monophosphate Kinase; Adenylate Kinase Isoenzyme 1; ATP-AMP Transphosphorylase 1; ATP:AMP Phosphotransferase; EC 2.7.4.3; Myokinase; Testis Secretory Sperm Binding Protein Li 58j; Epididymis Secretory Sperm Binding Protein; EC 2.7.4.10; EC 2.7.4.6; HTL-S-58j; EC 2.7.4; AK 1
Background	Gene Name: AK1 NCBI Gene Entry: 203 UniProt Entry: P00568
Application Information	Molecular Weight: Predicted, 45 kDa; observed, 44 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB985 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human Adenylate Kinase 1
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Adenylate kinase 1 expression in HepG2 cells using anti-Adenylate kinase 1 antibody (Cat#5329, 1:2,000). Green, isotype control; red,Adenylate kinase 1 .
Immunocytochemical staining of HepG2 cells with anti-Adenylate kinase 1 antibody (Cat#5329, 1:1,000). Nuclei were stained blue with DAPI; Adenylate kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-adenylate kinase 1 antibody (Cat#5329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-adenylate kinase 1 antibody (Cat#5329, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

AK1
货号：

5329
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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