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Anti-AKR1A1 Mouse Monoclonal Antibody #5332

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Flow cytometric analysis of AKR1A1 expression in HepG2 cells using anti-AKR1A1 antibody (Cat#5332, 1:2,000). Green, isotype control; red, AKR1A1.

Immunocytochemical staining of HepG2 cells with anti-AKR1A1 antibody (Cat#5332, 1:1,000). Nuclei were stained blue with DAPI; AKR1A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-AKR1A1 antibody (Cat#5332). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKR1A1 antibody (Cat#5332, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

AKR1A1
货号：

5332
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-AKR1A1 Mouse Monoclonal Antibody
Aliases	AKR1A1; Aldo-Keto Reductase Family 1 Member A1; ALR; Aldehyde Reductase; DD3; Dihydrodiol Dehydrogenase 3; Glucuronolactone Reductase; Glucuronate Reductase; EC 1.1.1.2; ALDR1; Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase); Epididymis Secretory Sperm Binding Protein Li 165mP; Epididymis Secretory Protein Li 6; Alcohol Dehydrogenase [NADP(+)]; Alcohol Dehydrogenase; EC 1.1.1.372; HEL-S-165mP; EC 1.1.1.54; EC 1.1.1.19; EC 1.1.1.20; EC 1.1.1; HEL-S-6; ARM
Background	Gene Name: AKR1A1 NCBI Gene Entry: 10327 UniProt Entry: P14550
Application Information	Molecular Weight: Predicted, 37 kDa; observed, 37 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB1000 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human AKR1A1
Isotype	Mouse IgG2b
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of AKR1A1 expression in HepG2 cells using anti-AKR1A1 antibody (Cat#5332, 1:2,000). Green, isotype control; red, AKR1A1.
Immunocytochemical staining of HepG2 cells with anti-AKR1A1 antibody (Cat#5332, 1:1,000). Nuclei were stained blue with DAPI; AKR1A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-AKR1A1 antibody (Cat#5332). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKR1A1 antibody (Cat#5332, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

AKR1A1
货号：

5332
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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