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Anti-NIMA Related Kinase 9 Mouse Monoclonal Antibody #5338

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Flow cytometric analysis of NIMA related kinase 9 expression in HeLa cells using anti-NIMA related kinase 9 antibody (Cat#5338, 1:2,000). Green, isotype control; red, NIMA related kinase 9.
Immunocytochemical staining of HeLa cells with anti-NIMA related kinase 9 antibody (Cat#5338, 1:1,000). Nuclei were stained blue with DAPI; NIMA related kinase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NIMA related kinase 9 antibody (Cat#5338). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIMA related kinase 9 antibody (Cat#5338, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    NEK9
  • 货号:
    5338
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-NIMA Related Kinase 9 Mouse Monoclonal Antibody
Aliases NEK9; NIMA Related Kinase 9; NERCC; NERCC1; Nek8; NIMA (Never In Mitosis Gene A)- Related Kinase 9; Serine/Threonine-Protein Kinase Nek9; NimA-Related Protein Kinase 9; DKFZp434D0935; Nercc1 Kinase; EC 2.7.11.1; MGC16714 2 5Never In Mitosis A-Related Kinase 9; NimA-Related Kinase 8; KIAA1995; LCCS10; APUG; NEK8; NC
Background

Gene Name: NEK9

NCBI Gene Entry: 91754

UniProt Entry: Q8TD19

Application Information

Molecular Weight: Predicted, 107 kDa; observed, 107 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB1030

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human NEK9
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NIMA related kinase 9 expression in HeLa cells using anti-NIMA related kinase 9 antibody (Cat#5338, 1:2,000). Green, isotype control; red, NIMA related kinase 9.
  • Immunocytochemical staining of HeLa cells with anti-NIMA related kinase 9 antibody (Cat#5338, 1:1,000). Nuclei were stained blue with DAPI; NIMA related kinase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-NIMA related kinase 9 antibody (Cat#5338). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIMA related kinase 9 antibody (Cat#5338, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of NIMA related kinase 9 expression in HeLa cells using anti-NIMA related kinase 9 antibody (Cat#5338, 1:2,000). Green, isotype control; red, NIMA related kinase 9.
Immunocytochemical staining of HeLa cells with anti-NIMA related kinase 9 antibody (Cat#5338, 1:1,000). Nuclei were stained blue with DAPI; NIMA related kinase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NIMA related kinase 9 antibody (Cat#5338). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIMA related kinase 9 antibody (Cat#5338, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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