Flow cytometric analysis of Cell division cycle associated 8 expression in HAP-1 cells using anti-Cell division cycle associated 8 antibody (Cat#5350, 1:2,000). Green, isotype control; red, Cell division cycle associated 8.
Immunocytochemical staining of HAP-1 cells with anti-Cell division cycle associated 8 antibody (Cat#5350, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle associated 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-cell division cycle associated 8 antibody (Cat#5350). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cell division cycle associated 8 antibody (Cat#5350, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
