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Anti-EIF4E2 Mouse Monoclonal Antibody #5408

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Flow cytometric analysis of EIF4E2 expression in HepG2 cells using anti-EIF4E2 antibody (Cat#5408, 1:1,000). Green, isotype control; red, EIF4E2.

Immunocytochemical staining of HepG2 cells with anti-EIF4E2 antibody (Cat#5408, 1:1,000). Nuclei were stained blue with DAPI; EIF4E2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-EIF4E2 antibody (Cat#5408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF4E2 antibody (Cat#5408, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

EIF4E2
货号：

5408
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-EIF4E2 Mouse Monoclonal Antibody
Aliases	EIF4E2; Eukaryotic Translation Initiation Factor 4E Family Member 2; Eukaryotic Translation Initiation Factor 4E-Like 3; EIF4EL3; IF4e; 4EHP; Eukaryotic Translation Initiation Factor 4E Homologous Protein; Eukaryotic Translation Initiation Factor 4E Type 2; MRNA Cap-Binding Protein Type 3; EIF4E-Like Protein 4E-LP; EIF-4E Type 2; H4EHP; EIF4E-Like Cap-Binding Protein; MRNA Cap-Binding Protein 4EHP; EIF4E Type 2; 4E-LP
Background	Gene Name: EIF4E2 NCBI Gene Entry: 9470 UniProt Entry: O60573
Application Information	Molecular Weight: Predicted, 28 kDa; observed, 28 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB1370 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human EIF4E2
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:1,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of EIF4E2 expression in HepG2 cells using anti-EIF4E2 antibody (Cat#5408, 1:1,000). Green, isotype control; red, EIF4E2.
Immunocytochemical staining of HepG2 cells with anti-EIF4E2 antibody (Cat#5408, 1:1,000). Nuclei were stained blue with DAPI; EIF4E2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-EIF4E2 antibody (Cat#5408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF4E2 antibody (Cat#5408, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

EIF4E2
货号：

5408
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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