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Anti-PTPRC Rabbit Monoclonal Antibody #5439

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Flow cytometric analysis of CD45 expression in HepG2 cells using anti-CD45 antibody (Cat#5439, 1:2,000). Green, isotype control; red, CD45.
Western blotting analysis using anti-CD45 antibody (Cat#5439). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD45 antibody (Cat#5439, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    PTPRC
  • 货号:
    5439
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-PTPRC Rabbit Monoclonal Antibody
Aliases PTPRC; Protein Tyrosine Phosphatase Receptor Type C; T200; GP180; CD45R; B220; CD45; LCA; LY5; Receptor-Type Tyrosine-Protein Phosphatase C; Glycoprotein 180; CD45 Antigen; L-CA; Protein Tyrosine Phosphatase, Receptor Type, C Polypeptide; T200 Leukocyte Common Antigen; Leukocyte-Common Antigen; Leukocyte Common Antigen; T200 Glycoprotein; EC 3.1.3.48; IMD105
Background

Gene Name: PTPRC

NCBI Gene Entry: 5788

UniProt Entry: P08575

Application Information

Molecular Weight: Predicted, 147 kDa; observed, 150-200 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1560

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PTPRC
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CD45 expression in HepG2 cells using anti-CD45 antibody (Cat#5439, 1:2,000). Green, isotype control; red, CD45.
  • Western blotting analysis using anti-CD45 antibody (Cat#5439). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD45 antibody (Cat#5439, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of CD45 expression in HepG2 cells using anti-CD45 antibody (Cat#5439, 1:2,000). Green, isotype control; red, CD45.
Western blotting analysis using anti-CD45 antibody (Cat#5439). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD45 antibody (Cat#5439, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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