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Anti-CRTC1 Rabbit Monoclonal Antibody #5514

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Flow cytometric analysis of CRTC1 expression in HepG2 cells using anti-CRTC1 antibody (Cat#5514, 1:2,000). Green, isotype control; red, CRTC1.

Immunocytochemical staining of HepG2 cells with anti-CRTC1 antibody (Cat#5514, 1:1,000) . Nuclei were stained blue with DAPI; CRTC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-CRTC1 antibody (Cat#5514). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRTC1 antibody (Cat#5514, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CRTC1
货号：

5514
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-CRTC1 Rabbit Monoclonal Antibody
Aliases	CRTC1; CREB Regulated Transcription Coactivator 1; TORC1; KIAA0616; MECT1; Mucoepidermoid Carcinoma Translocated Protein 1; CREB-Regulated Transcription Coactivator 1; FLJ14027; TORC-1; WAMTP1; Transducer Of Regulated CAMP Response Element-Binding Protein (CREB) 1; Transducer Of Regulated CAMP Response Element-Binding Protein 1; Transducer Of Regulated CAMP Response Element-Binding Protein; Mucoepidermoid Carcinoma Translocated 1; Transducer Of CREB Protein 1; Mastermind-Like Protein 2; MAML2; Mam-2
Background	Gene Name: CRTC1 NCBI Gene Entry: 23373 UniProt Entry: Q6UUV9
Application Information	Molecular Weight: Predicted, 67 kDa; observed, 68-90 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB1850 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CRTC1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CRTC1 expression in HepG2 cells using anti-CRTC1 antibody (Cat#5514, 1:2,000). Green, isotype control; red, CRTC1.
Immunocytochemical staining of HepG2 cells with anti-CRTC1 antibody (Cat#5514, 1:1,000) . Nuclei were stained blue with DAPI; CRTC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CRTC1 antibody (Cat#5514). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRTC1 antibody (Cat#5514, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CRTC1
货号：

5514
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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