Anti-Aurora Kinase A Rabbit Monoclonal Antibody - 产品中心

Anti-Aurora Kinase A Rabbit Monoclonal Antibody #5529

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Flow cytometric analysis of Aurora kinase A expression in HepG2 cells using anti-Aurora kinase A antibody (Cat#5529, 1:2,000). Green, isotype control; red, Aurora kinase A.

Immunocytochemical staining of HepG2 cells with anti-Aurora kinase A antibody (Cat#5529, 1:1,000) . Nuclei were stained blue with DAPI; Aurora kinase A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-aurora kinase A antibody (Cat#5529). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aurora kinase A antibody (Cat#5529, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

AURKA
货号：

5529
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Aurora Kinase A Rabbit Monoclonal Antibody
Aliases	AURKA; Aurora Kinase A; BTAK; ARK1; AIK; PPP1R47; STK15; AurA; STK7; STK6; Protein Phosphatase 1, Regulatory Subunit 47; Serine/Threonine-Protein Kinase Aurora-A; Serine/Threonine-Protein Kinase 6; Breast Tumor-Amplified Kinase; Aurora/IPL1-Related Kinase 1; Aurora 2; Serine/Threonine-Protein Kinase Ayk1; Serine/Threonine Protein Kinase 15; Serine/Threonine-Protein Kinase 15; Ipl1- And Aurora-Related Kinase 1; Serine/Threonine Kinase 15; Serine/Threonine Kinase 6; Aurora/IPL1-Like Kinase; Aurora-Related Kinase 1; Aurora-A Kinase; EC 2.7.11.1; AIRK1; ARK-1; AURA; AYK1; IAK1
Background	Gene Name: AURKA NCBI Gene Entry: 6790 UniProt Entry: O14965
Application Information	Molecular Weight: Predicted, 46 kDa; observed, 45 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB1925 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Aurora kinase A
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Aurora kinase A expression in HepG2 cells using anti-Aurora kinase A antibody (Cat#5529, 1:2,000). Green, isotype control; red, Aurora kinase A.
Immunocytochemical staining of HepG2 cells with anti-Aurora kinase A antibody (Cat#5529, 1:1,000) . Nuclei were stained blue with DAPI; Aurora kinase A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-aurora kinase A antibody (Cat#5529). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aurora kinase A antibody (Cat#5529, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

AURKA
货号：

5529
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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