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Anti-ITPR1 Rabbit Monoclonal Antibody #5530

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Flow cytometric analysis of ITPR1 expression in H9c2 cells using anti-ITPR1 antibody (Cat#5530, 1:2,000). Green, isotype control; red, ITPR1.
Immunocytochemical staining of H9C2 cells with anti-ITPR1 antibody (Cat#5530, 1:1,000) . Nuclei were stained blue with DAPI; ITPR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ITPR1 antibody (Cat#5530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ITPR1 antibody (Cat#5530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    ITPR1
  • 货号:
    5530
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-ITPR1 Rabbit Monoclonal Antibody
Aliases ITPR1; Inositol 1,4,5-Trisphosphate Receptor Type 1; Insp3r1; PPP1R94; IP3R1; ACV; Inositol 1,4,5-Trisphosphate-Gated Calcium Channel ITPR1; Protein Phosphatase 1, Regulatory Subunit 94; Type 1 Inositol 1,4,5-Trisphosphate Receptor; Inositol 1,4,5 Trisphosphate Receptor; Type 1 InsP3 Receptor; IP3R 1; SCA15; SCA16; SCA29; Inositol 1,4,5-Trisphosphate Receptor, Type 1; Inositol 1,4,5-Triphosphate Receptor, Type 1; Spinocerebellar Ataxia 15; Spinocerebellar Ataxia 16; Spinocerebellar Ataxia 29; IP3 Receptor Isoform 1; IP3 Receptor; INSP3R1; InsP3R1; CLA4; IP3R
Background

Gene Name: ITPR1

NCBI Gene Entry: 3708

UniProt Entry: Q14643

Application Information

Molecular Weight: Predicted, 314 kDa; observed, 314 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1930

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human ITPR1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ITPR1 expression in H9c2 cells using anti-ITPR1 antibody (Cat#5530, 1:2,000). Green, isotype control; red, ITPR1.
  • Immunocytochemical staining of H9C2 cells with anti-ITPR1 antibody (Cat#5530, 1:1,000) . Nuclei were stained blue with DAPI; ITPR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-ITPR1 antibody (Cat#5530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ITPR1 antibody (Cat#5530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of ITPR1 expression in H9c2 cells using anti-ITPR1 antibody (Cat#5530, 1:2,000). Green, isotype control; red, ITPR1.
Immunocytochemical staining of H9C2 cells with anti-ITPR1 antibody (Cat#5530, 1:1,000) . Nuclei were stained blue with DAPI; ITPR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ITPR1 antibody (Cat#5530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ITPR1 antibody (Cat#5530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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