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Anti-UBE2D3 Rabbit Monoclonal Antibody #5624

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Western blotting analysis using anti-UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti-UBE2D3 antibody (Cat#5624, 1:1,000) . Nuclei were stained blue with DAPI; UBE2D3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2D3 expression in C2C12 cells using anti-UBE2D3 antibody (Cat#5624, 1:2,000). Green, isotype control; red, UBE2D3.
  • 基因名：
    UBE2D3
  • 货号：
    5624
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-UBE2D3 Rabbit Monoclonal Antibody
Aliases UBE2D3; Ubiquitin Conjugating Enzyme E2 D3; UbcH5C; Ubiquitin-Conjugating Enzyme E2D 3 (Homologous To Yeast UBC4/5); Ubiquitin-Conjugating Enzyme E2D 3 (UBC4/5 Homolog, Yeast); (E3-Independent) E2 Ubiquitin-Conjugating Enzyme D3; Ubiquitin-Conjugating Enzyme E2-17 KDa 3; Ubiquitin-Conjugating Enzyme E2(17)KB 3; Ubiquitin-Conjugating Enzyme E2 D3; E2 Ubiquitin-Conjugating Enzyme D3; Ubiquitin Carrier Protein D3; Ubiquitin-Protein Ligase D3; Ubiquitin-Conjugating Enzyme E2D 3; Ubiquitin Conjugating Enzyme E2D 3; EC 2.3.2.23; EC 2.3.2.24; EC 6.3.2.19; E2(17)KB3; UBC4/5; UBCH5C; UBC5C
Background

Gene Name: UBE2D3

NCBI Gene Entry: 7323

UniProt Entry: P61077
Application Information

Molecular Weight: Predicted, 17 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4575

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human UBE2D3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with anti-UBE2D3 antibody (Cat#5624, 1:1,000) . Nuclei were stained blue with DAPI; UBE2D3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of UBE2D3 expression in C2C12 cells using anti-UBE2D3 antibody (Cat#5624, 1:2,000). Green, isotype control; red, UBE2D3.
Citation(0)
  • 基因名：
    UBE2D3
  • 货号：
    5624
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Western blotting analysis using anti-UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti-UBE2D3 antibody (Cat#5624, 1:1,000) . Nuclei were stained blue with DAPI; UBE2D3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-UBE2D3 antibody (Cat#5624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D3 antibody (Cat#5624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2D3 expression in C2C12 cells using anti-UBE2D3 antibody (Cat#5624, 1:2,000). Green, isotype control; red, UBE2D3.
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