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Anti-WASF1 Rabbit Monoclonal Antibody #5630

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Western blotting analysis using anti-WASF1 antibody (Cat#5630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WASF1 antibody (Cat#5630, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti- antibody (Cat#5630, 1:1,000) . Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-WASF1 antibody (Cat#5630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WASF1 antibody (Cat#5630, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of WASF1 expression in C2C12 cells using anti-WASF1 antibody (Cat#5630, 1:2,000). Green, isotype control; red, WASF1.
  • 基因名：
    WASF1
  • 货号：
    5630
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-WASF1 Rabbit Monoclonal Antibody
Aliases WASF1; WASP Family Member 1; WAVE1; SCAR1; KIAA0269; WAVE; Wiskott-Aldrich Syndrome Protein Family Member 1; Verprolin Homology Domain-Containing Protein 1; WASP Family Protein Member 1; WAS Protein Family Member 1; Actin-Binding Protein WASF1; Protein WAVE-1; Homology Of Dictyostelium Scar 1; WAS Protein Family, Member 1; NEDALVS
Background

Gene Name: WASF1

NCBI Gene Entry: 8936

UniProt Entry: Q92558
Application Information

Molecular Weight: Predicted, 62 kDa; observed, 80 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4605

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen Recombinant protein of human WASF1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-WASF1 antibody (Cat#5630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WASF1 antibody (Cat#5630, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with anti- antibody (Cat#5630, 1:1,000) . Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-WASF1 antibody (Cat#5630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WASF1 antibody (Cat#5630, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of WASF1 expression in C2C12 cells using anti-WASF1 antibody (Cat#5630, 1:2,000). Green, isotype control; red, WASF1.
Citation(0)
  • 基因名：
    WASF1
  • 货号：
    5630
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Western blotting analysis using anti-WASF1 antibody (Cat#5630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WASF1 antibody (Cat#5630, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti- antibody (Cat#5630, 1:1,000) . Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-WASF1 antibody (Cat#5630). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WASF1 antibody (Cat#5630, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of WASF1 expression in C2C12 cells using anti-WASF1 antibody (Cat#5630, 1:2,000). Green, isotype control; red, WASF1.
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