Flow cytometric analysis of cyclin dependent kinase 5 expression in HepG2 cells using cyclin dependent kinase 5 antibody (Cat#61114, 1:2,000). Green, isotype control; red, cyclin dependent kinase 5.
Immunocytochemical staining of HepG2 cells with cyclin dependent kinase 5 antibody (Cat#61114, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-cyclin dependent kinase 5 antibody (Cat#61114). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase 5 antibody (Cat#61114, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cyclin dependent kinase 5 antibody (Cat#61114). Cyclin dependent kinase 5 expression in wild type (WT) and cyclin dependent kinase 5 (CDK5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-cyclin dependent kinase 5 antibody (Cat#61114, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
