Flow cytometric analysis of Peptidylprolyl isomerase B expression in H9c2 cells using Peptidylprolyl isomerase B antibody (Cat#61118, 1:2,000). Green, isotype control; red, Peptidylprolyl isomerase B.
Immunocytochemical staining of H9C2 cells with anti-Peptidylprolyl isomerase B antibody (Cat #61118, 1:1,000) . Nuclei were stained blue with DAPI; Peptidylprolyl isomerase B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Peptidylprolyl isomerase B antibody (Cat#61118). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peptidylprolyl isomerase B antibody (Cat#61118, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Peptidylprolyl isomerase B antibody (Cat#61118). Peptidylprolyl isomerase B expression in wild type (WT) and peptidylprolyl isomerase B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Peptidylprolyl isomerase B antibody (Cat#61118, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
