Flow cytometric analysis of CDKN2C expression in HeLa cells using CDKN2C antibody(Cat#61139, 1:2,000). Green, isotype control; red, CDKN2C.
Immunocytochemical staining of Hela cells with anti-CDKN2C antibody (Cat#61139, 1:1,000). Nuclei were stained blue with DAPI; CDKN2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CDKN2C antibody (Cat#61139). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDKN2C antibody (Cat#61139, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CDKN2C antibody (Cat#61139). CDKN2C expression in wild type (WT) and CDKN2C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CDKN2C antibody (Cat#61139, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of CDKN2C knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-CDKN2C antibody (Cat#61139, 1:2,000) and analyzed using BD flow cytometer.
