Immunocytochemical staining of HAP1 cells with anti-glycophorin C antibody (Cat#61143, 1:1,000). Nuclei were stained blue with DAPI; Glycophorin C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-glycophorin C antibody (Cat#61143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glycophorin C antibody (Cat#61143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-glycophorin C antibody (Cat#61143). Glycophorin C expression in wild-type (WT) and GYPC shRNA knockdown (KD) HAP-1 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-glycophorin C antibody (Cat#61143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). GYPC, glycophorin C (Gerbich blood group).
Validation of Glycophorin C knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with anti-Glycophorin C antibody (Cat#61143, 1:2,000) and analyzed using BD flow cytometer.
