Flow cytometric analysis of leucine aminopeptidase 3 expression in HAP-1 cells using anti-Leucine aminopeptidase 3 antibody (Cat#61144, 1:2,000). Green, isotype control; red, leucine aminopeptidase 3.
Immunocytochemical staining of HAP-1 cells with anti-leucine aminopeptidase 3 antibody (Cat#61144, 1:1,000). Nuclei were stained blue with DAPI; Leucine aminopeptidase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-leucine aminopeptidase 3 antibody (Cat#61144). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-leucine aminopeptidase 3 antibody (Cat#61144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-leucine aminopeptidase 3 antibody (Cat#61144). Leucine aminopeptidase 3 expression in wild type (WT) and leucine aminopeptidase 3 (LAP3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-leucine aminopeptidase 3 antibody (Cat#61144, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Leucine aminopeptidase 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Leucine aminopeptidase 3 antibody (Cat#61144, 1:2,000) and analyzed using BD flow cytometer.
