Flow cytometric analysis of MHC class 1 expression in HeLa cells using MHC class 1 antibody (Cat#61166, 1:2,000). Green, isotype control; red, MHC class 1.
Immunocytochemical staining of HAP-1 cells with anti-MHC class 1 antibody (Cat#61166, 1:1,000). Nuclei were stained blue with DAPI; MHC class 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High . Scale bar, 20 μm.
Western blotting analysis using anti-MHC class 1 antibody (Cat#61166). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MHC class 1 antibody (Cat#61166, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-MHC class 1 antibody (Cat#61166). MHC class 1 expression in wild type (WT) and HLA-A shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MHC class 1 antibody (Cat#61166, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). HLA-A, Major Histocompatibility Complex, Class I.
Validation of MHC class 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with anti-MHC class 1 antibody (Cat#61166, 1:2,000) and analyzed using BD flow cytometer.
