Flow cytometric analysis of prohibitin expression in HT-1080 cells using prohibitin antibody (Cat#61170, 1:2,000). Green, isotype control; red, prohibitin.
Immunocytochemical staining of HT-1080 cells with prohibitin antibody (Cat#61170, 1:1,000). Nuclei were stained blue with DAPI; Prohibitin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-prohibitin antibody (Cat#61170). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-prohibitin antibody (Cat#61170, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-prohibitin antibody (Cat#61170). Prohibitin expression in wild type (WT) and prohibitin (PHB1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-prohibitin antibody (Cat#61170, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-Prohibitin antibody (Cat#61170, 1:1,000), Top panel: wild-type (WT); Bottom panal: Prohibitin shRNA knockdown (KD). Nuclei were stained blue with DAPI; Prohibitin was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
