Flow cytometric analysis of Crystallin alpha B expression in H9c2 cells using crystallin alpha B antibody (Cat#61176, 1:2,000). Green, isotype control; red, crystallin alpha B.
Immunocytochemical staining of HT-1080 cells with crystallin alpha B antibody (Cat#61176, 1:1,000). Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-crystallin alpha B antibody (Cat#61176). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-crystallin alpha B antibody (Cat#61176, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-crystallin α B antibody (Cat#61176). Crystallin α B expression in wild type (WT) and crystallin α B (CRYAB) shRNA knockdown (KD) H9c2 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-crystallin α B antibody (Cat#61176, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
