Flow cytometric analysis of USP9X expression in HepG2 cells using USP9X antibody (Cat#61180, 1:2,000). Green, isotype control; red, USP9X.
Immunocytochemical staining of HepG2 cells with USP9X antibody (Cat#61180, 1:1,000). Nuclei were stained blue with DAPI; USP9X was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-USP9X antibody (Cat#61180). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-USP9X antibody (Cat#61180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-USP9X antibody (Cat#61180). USP9X expression in wild-type (WT) and USP9X shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-USP9X antibody (Cat#61180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Immunocytochemical staining of HeLa cells using anti-USP9X antibody (Cat#61180, 1:1,000), Top panel: wild-type (WT); Bottom panal: USP9X shRNA knockdown (KD). Nuclei were stained blue with DAPI; USP9X was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.