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KD-Validated Anti-Inhibin subunit beta B Rabbit Monoclonal Antibody #61184

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Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#61184, 1:2,000). Green, isotype control; red, inhibin subunit beta B.
Immunocytochemical staining of HT-1080 cells with anti-inhibin subunit beta B antibody (Cat#61184, 1:1,000). Nuclei were stained blue with DAPI; Inhibin subunit beta B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#61184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#61184, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#61184). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#61184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    INHBB
  • 货号：
    61184
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Inhibin subunit beta B Rabbit Monoclonal Antibody
Aliases INHBB; Inhibin Subunit Beta B; Inhibin Beta B Chain; Activin Beta-B Chain; Inhibin, Beta B (Activin AB Beta Polypeptide); Activin AB Beta Polypeptide; Inhibin Beta B Subunit; Inhibin, Beta B; Inhibin, Beta-2
Background

Gene Name: INHBB

NCBI Gene Entry: 3625

UniProt Entry: P09529
Application Information

Molecular Weight: Predicted, 45 kDa, observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6100

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Inhibin beta B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#61184, 1:2,000). Green, isotype control; red, inhibin subunit beta B.
  • Immunocytochemical staining of HT-1080 cells with anti-inhibin subunit beta B antibody (Cat#61184, 1:1,000). Nuclei were stained blue with DAPI; Inhibin subunit beta B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#61184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#61184, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#61184). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#61184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    INHBB
  • 货号：
    61184
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of inhibin subunit beta B expression in HT-1080 cells using inhibin subunit beta B antibody (Cat#61184, 1:2,000). Green, isotype control; red, inhibin subunit beta B.
Immunocytochemical staining of HT-1080 cells with anti-inhibin subunit beta B antibody (Cat#61184, 1:1,000). Nuclei were stained blue with DAPI; Inhibin subunit beta B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#61184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#61184, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-inhibin subunit beta B antibody (Cat#61184). Inhibin subunit beta B expression in wild-type (WT) and inhibin subunit beta B (INHBB) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-inhibin subunit beta B antibody (Cat#61184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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