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KD-Validated Anti-LEFTY1 + LEFTY2 Rabbit Monoclonal Antibody #61190

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Immunocytochemical staining of HepG2 cells with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:1,000). Nuclei were stained blue with DAPI; LEFTY1 + LEFTY2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#61190). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#61190). LEFTY1 + LEFTY2 expression in wild type (WT) and LEFTY1 + LEFTY2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    LEFTY2
  • 货号：
    61190
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-LEFTY1 + LEFTY2 Rabbit Monoclonal Antibody
Aliases LEFTY2; Left-Right Determination Factor 2; LEFTYA; LEFTA; TGFB4; EBAF; Endometrial Bleeding-Associated Factor; Left-Right Determination Factor A; Transforming Growth Factor Beta-4; Protein Lefty-2; Protein Lefty-A; TGF-Beta-4; Endometrial Bleeding Associated Factor (Left-Right Determination, Factor A; Transforming Growth Factor Beta Superfamily); Transforming Growth Factor, Beta-4 (Endometrial Bleeding-Associated Factor; LEFTY A)
Background

Gene Name: LEFTY2

NCBI Gene Entry: 7044

UniProt Entry: O00292
Application Information

Molecular Weight: Predicted, 41 kDa, observed, 34 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6115

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human LEFTY1 + LEFTY2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HepG2 cells with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:1,000). Nuclei were stained blue with DAPI; LEFTY1 + LEFTY2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#61190). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#61190). LEFTY1 + LEFTY2 expression in wild type (WT) and LEFTY1 + LEFTY2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    LEFTY2
  • 货号：
    61190
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Immunocytochemical staining of HepG2 cells with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:1,000). Nuclei were stained blue with DAPI; LEFTY1 + LEFTY2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#61190). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#61190). LEFTY1 + LEFTY2 expression in wild type (WT) and LEFTY1 + LEFTY2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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