Flow cytometric analysis of Peptidylprolyl isomerase F expression in C2C12 cells using Peptidylprolyl isomerase F antibody (Cat#61192, 1:2,000). Green, isotype control; red, Peptidylprolyl isomerase F.
Immunocytochemical staining of C2C12 cells with Peptidylprolyl isomerase F antibody (Cat#61192, 1:1,000). Nuclei were stained blue with DAPI; Peptidylprolyl isomerase F was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Peptidylprolyl isomerase F antibody (Cat#61192). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peptidylprolyl isomerase F antibody (Cat#61192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Peptidylprolyl isomerase F antibody (Cat#61192). Peptidylprolyl isomerase F expression in wild type (WT) and peptidylprolyl isomerase F shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Peptidylprolyl isomerase F antibody (Cat#61192, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
