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KD-Validated Anti-RAC3 Rabbit Monoclonal Antibody #61199

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Flow cytometric analysis of RAC3 expression in HAP-1 cells using
RAC3 antibody (Cat#61199, 1:2,000). Green, isotype control; red, RAC3.

Immunocytochemical staining of HAP-1 cells with RAC3 antibody (Cat#61199, 1:1,000). Nuclei were stained blue with DAPI; RAC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-RAC3 antibody (Cat#61199). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMB10 antibody (Cat#61199, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-RAC3 antibody (Cat#61199). RAC3 expression in wild type (WT) and RAC3 shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAC3 antibody (Cat#61199, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

RAC3
货号：

61199
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-RAC3 Rabbit Monoclonal Antibody
Aliases	RAC3; Rac Family Small GTPase 3; Ras-Related C3 Botulinum Toxin Substrate 3 (Rho Family, Small GTP Binding Protein Rac3); Ras-Related C3 Botulinum Toxin Substrate 3; P21-Rac3; Rho Family, Small GTP Binding Protein Rac3; EC 3.6.5.2
Background	Gene Name: RAC3 NCBI Gene Entry: 5881 UniProt Entry: P60763
Application Information	Molecular Weight: Predicted, 21 kDa, observed, 21 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2175 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human RAC3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of RAC3 expression in HAP-1 cells using
RAC3 antibody (Cat#61199, 1:2,000). Green, isotype control; red, RAC3.
Immunocytochemical staining of HAP-1 cells with RAC3 antibody (Cat#61199, 1:1,000). Nuclei were stained blue with DAPI; RAC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-RAC3 antibody (Cat#61199). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMB10 antibody (Cat#61199, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RAC3 antibody (Cat#61199). RAC3 expression in wild type (WT) and RAC3 shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAC3 antibody (Cat#61199, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

RAC3
货号：

61199
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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