Flow cytometric analysis of IQGAP1 expression in HepG2 cells using IQGAP1 antibody (Cat#61278, 1:2,000). Green, isotype control; red, IQGAP1.
lmmunocytochemical staining of HepG2 cells with lQGAP1 antibody (Cat#61278, 1:1,000). Nuclei were stained blue with DAPl; IQGAP1 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-IQGAP1 antibody (Cat#61278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IQGAP1 antibody (Cat#61278, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-IQGAP1 antibody (Cat#61278). IQGAP1 expression in wild-type (WT) and IQGAP1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IQGAP1 antibody (Cat#61278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of IQGAP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with anti-IQGAP1 antibody (Cat#61278, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HepG2 cells using anti-IQGAP1 antibody (Cat#61278, 1:1,000), Top panel: wild-type (WT); Bottom panal: IQGAP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; IQGAP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
