Flow cytometric analysis of Aurora kinase B expression in HAP-1 cells using Aurora kinase B antibody (Cat#61330, 1:2,000). Green, isotype control; red, Aurora kinase B.
Immunocytochemical staining of HAP-1 cells with anti-Aurora kinase B antibody (Cat#61330, 1:1,000). Nuclei were stained blue with DAPI; Aurora kinase B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-aurora kinase B antibody (Cat#61330). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aurora kinase B antibody (Cat#61330, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-aurora kinase B antibody (Cat#61330). Aurora kinase B expression in wild-type (WT) and aurora kinase B (AURKB) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-aurora kinase B antibody (Cat#61330, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
