Flow cytometric analysis of CD44 expression in HT-1080 cells using CD44 antibody (Cat#61350, 1:2,000). Green, isotype control; red, CD44.
Immunocytochemical staining of HT-1080 cells with CD44 antibody (Cat#61350, 1:1,000). Nuclei were stained blue with DAPI; CD44 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-CD44 antibody (Cat#61350). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD44 antibody (Cat#61350, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-CD44 antibody (Cat#61350). CD44 expression in wild type (WT) and CD44 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD44 antibody (Cat#61350, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-CD44 antibody (Cat#61350, 1:1,000), Top panel: wild-type (WT); Bottom panal: CD44 shRNA knockdown (KD). Nuclei were stained blue with DAPI; CD44 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
