Flow cytometric analysis of Cathepsin D expression in HT-1080 cells using anti-Cathepsin D antibody (Cat#61363, 1:2,000). Green, isotype control; red, Cathepsin D.
Immunocytochemical staining of HT-1080 cells with anti-Cathepsin D antibody (Cat#61363, 1:1,000) . Nuclei were stained blue with DAPI; Cathepsin D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Cathepsin D antibody (Cat#61363). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cathepsin D antibody (Cat#61363, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Cathepsin D antibody (Cat#61363). Cathepsin D expression in wild type (WT) and Cathepsin D shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cathepsin D antibody (Cat#61363, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
