Flow cytometric analysis of Aly/REF export factor expression in HeLa cells using Aly/REF export factor antibody (Cat#61394, 1:2,000). Green, isotype control; red, Aly/REF export factor.
Immunocytochemical staining of Hela cells with anti-Aly/REF export factor antibody (Cat#61394, 1:1,000). Nuclei were stained blue with DAPI; Aly/REF export factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Aly/REF export factor antibody (Cat#61394). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aly/REF export factor antibody (Cat#61394, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Aly/REF export factor antibody (Cat#61394). Aly/REF export factor expression in wild-type (WT) and Aly/REF export factor (ALYREF) knockdown (KD) HeLa cells with 25 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Aly/REF export factor antibody (Cat#61394, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).