Flow cytometric analysis of Histone deacetylase 8 expression in HeLa cells using Histone deacetylase 8 antibody (Cat#61423, 1:2,000). Green, isotype control; red, Histone deacetylase 8.
Immunocytochemical staining of HeLa cells with anti-Histone deacetylase 8 antibody (Cat#61423, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-histone deacetylase 8 antibody (Cat#61423). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone deacetylase 8 antibody (Cat#61423, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-histone deacetylase 8 antibody (Cat#61423). Histone deacetylase 8 expression in wild-type (WT) and histone deacetylase 8 (HDAC8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-histone deacetylase 8 antibody (Cat#61423, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Histone deacetylase 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Histone deacetylase 8 antibody (Cat#61423, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Histone deacetylase 8 antibody (Cat#61423, 1:1,000), Top panel: wild-type (WT); Bottom panal: Histone deacetylase 8 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
