Flow cytometric analysis of Core-binding factor subunit beta expression in HAP-1 cells using Core-binding factor subunit beta antibody (Cat#61442, 1:2,000). Green, isotype control; red, Core-binding factor subunit beta.
Immunocytochemical staining of C2C12 cells with anti-Core-binding factor subunit beta antibody (Cat#61442, 1:1,000) . Nuclei were stained blue with DAPI; Core-binding factor subunit beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Core-binding factor subunit beta antibody (Cat#61442). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Core-binding factor subunit beta antibody (Cat#61442, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Core-binding factor subunit beta antibody (Cat#61442). Core-binding factor subunit beta expression in wild type (WT) and Core-binding factor subunit beta shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Core-binding factor subunit beta antibody (Cat#61442, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
