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KD-Validated Anti-Cystathionine beta-synthase Rabbit Monoclonal Antibody #61446

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Flow cytometric analysis of Cystathionine beta-synthase expression in HeLa cells using Cystathionine beta-synthase antibody (Cat#61446, 1:2,000). Green, isotype control; red, Cystathionine beta-synthase.
Immunocytochemical staining of Hela cells with Cystathionine beta-synthase antibody (Cat#61446, 1:1,000). Nuclei were stained blue with DAPI; Cystathionine beta-synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Cystathionine beta-synthase antibody (Cat#61446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Cystathionine beta-synthase antibody (Cat#61446). Cystathionine beta-synthase expression in wild type (WT) and Cystathionine beta-synthase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Cystathionine beta-synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Aryl hydrocarbon receptor antibody (Cat#61446, 1:1,000), Top panel: wild-type (WT); Bottom panal:Aryl hydrocarbon receptor shRNA knockdown (KD). Nuclei were stained blue with DAPI;Aryl hydrocarbon receptor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名:
    CBS
  • 货号:
    61446
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Cystathionine beta-synthase Rabbit Monoclonal Antibody
Aliases CBS; Cystathionine Beta-Synthase; HIP4; Serine Sulfhydrase; Beta-Thionase; EC 4.2.1.22; Cystathionine Beta-Synthase-Like Protein; Cystathionine-Beta-Synthase; Methylcysteine Synthase; CBSL
Background

Gene Name: CBS

NCBI Gene Entry: 875

UniProt Entry: P35520

Application Information

Molecular Weight: Predicted, 61 kDa, observed, 61 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3800

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human CBS
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Cystathionine beta-synthase expression in HeLa cells using Cystathionine beta-synthase antibody (Cat#61446, 1:2,000). Green, isotype control; red, Cystathionine beta-synthase.
  • Immunocytochemical staining of Hela cells with Cystathionine beta-synthase antibody (Cat#61446, 1:1,000). Nuclei were stained blue with DAPI; Cystathionine beta-synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Cystathionine beta-synthase antibody (Cat#61446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Cystathionine beta-synthase antibody (Cat#61446). Cystathionine beta-synthase expression in wild type (WT) and Cystathionine beta-synthase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Cystathionine beta-synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Aryl hydrocarbon receptor antibody (Cat#61446, 1:1,000), Top panel: wild-type (WT); Bottom panal:Aryl hydrocarbon receptor shRNA knockdown (KD). Nuclei were stained blue with DAPI;Aryl hydrocarbon receptor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
Flow cytometric analysis of Cystathionine beta-synthase expression in HeLa cells using Cystathionine beta-synthase antibody (Cat#61446, 1:2,000). Green, isotype control; red, Cystathionine beta-synthase.
Immunocytochemical staining of Hela cells with Cystathionine beta-synthase antibody (Cat#61446, 1:1,000). Nuclei were stained blue with DAPI; Cystathionine beta-synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Cystathionine beta-synthase antibody (Cat#61446). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Cystathionine beta-synthase antibody (Cat#61446). Cystathionine beta-synthase expression in wild type (WT) and Cystathionine beta-synthase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Cystathionine beta-synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Cystathionine beta-synthase antibody (Cat#61446, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Aryl hydrocarbon receptor antibody (Cat#61446, 1:1,000), Top panel: wild-type (WT); Bottom panal:Aryl hydrocarbon receptor shRNA knockdown (KD). Nuclei were stained blue with DAPI;Aryl hydrocarbon receptor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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