Flow cytometric analysis of Dihydrolipoamide dehydrogenase expression in HT-1080 cells using Dihydrolipoamide dehydrogenase antibody (Cat#61456, 1:2,000). Green, isotype control; red, Dihydrolipoamide dehydrogenase.
Immunocytochemical staining of HT-1080 cells with anti-Dihydrolipoamide dehydrogenase antibody (Cat #61456, 1:1,000) . Nuclei were stained blue with DAPI; Dihydrolipoamide dehydrogenase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Dihydrolipoamide dehydrogenase antibody (Cat#61456). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Dihydrolipoamide dehydrogenase antibody (Cat#61456, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Dihydrolipoamide dehydrogenase antibody (Cat#61456). Dihydrolipoamide dehydrogenase expression in wild type (WT) and Dihydrolipoamide dehydrogenase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Dihydrolipoamide dehydrogenase antibody (Cat#61456, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Dihydrolipoamide dehydrogenase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Dihydrolipoamide dehydrogenase antibody (Cat#61456, 1:2,000) and analyzed using CytoFLEX.
