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KD-Validated Anti-Hsp60 Rabbit Monoclonal Antibody #61476

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Flow cytometric analysis of Hsp60 expression in HT-1080 cells using Hsp60 antibody (Cat#61476, 1:2,000). Green, isotype control; red, Hsp60.
Immunocytochemical staining of HT-1080 cells with Hsp60 antibody (Cat#61476, 1:1,000). Nuclei were stained blue with DAPI; Hsp60 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Hsp60 antibody (Cat#61476). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp60 antibody (Cat#61476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Hsp60 antibody (Cat#61476). Hsp60 expression in wild type (WT) and Hsp60 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Hsp60 antibody (Cat#61476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Hsp60 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Hsp60 antibody (Cat#61476, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Hsp60 antibody (Cat#61476, 1:1,000), Top panel: wild-type (WT); Bottom panal: Hsp60 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Hsp60 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    HSPD1
  • 货号：
    61476
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Hsp60 Rabbit Monoclonal Antibody
Aliases HSPD1; Heat Shock Protein Family D (Hsp60) Member 1; HSP60; 60 KDa Heat Shock Protein, Mitochondrial; Heat Shock 60kDa Protein 1 (Chaperonin); Mitochondrial Matrix Protein P1; P60 Lymphocyte Protein; 60 KDa Chaperonin; Chaperonin 60; HuCHA60; HSP-60; CPN60; GROEL; SPG13; Spastic Paraplegia 13 (Autosomal Dominant); Epididymis Secretory Sperm Binding Protein; Heat Shock 60kD Protein 1 (Chaperonin); Short Heat Shock Protein 60 Hsp60s1; Heat Shock Protein 65; Heat Shock Protein 60; EC 5.6.1.7; GroEL; HSP65; Hsp60; HLD4
Background

Gene Name: HSPD1

NCBI Gene Entry: 3329

UniProt Entry: P10809
Application Information

Molecular Weight: Predicted, 61 kDa, observed, 60 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2680

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Hsc60
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Hsp60 expression in HT-1080 cells using Hsp60 antibody (Cat#61476, 1:2,000). Green, isotype control; red, Hsp60.
  • Immunocytochemical staining of HT-1080 cells with Hsp60 antibody (Cat#61476, 1:1,000). Nuclei were stained blue with DAPI; Hsp60 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Hsp60 antibody (Cat#61476). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp60 antibody (Cat#61476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Hsp60 antibody (Cat#61476). Hsp60 expression in wild type (WT) and Hsp60 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Hsp60 antibody (Cat#61476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Hsp60 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Hsp60 antibody (Cat#61476, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Hsp60 antibody (Cat#61476, 1:1,000), Top panel: wild-type (WT); Bottom panal: Hsp60 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Hsp60 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    HSPD1
  • 货号：
    61476
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Hsp60 expression in HT-1080 cells using Hsp60 antibody (Cat#61476, 1:2,000). Green, isotype control; red, Hsp60.
Immunocytochemical staining of HT-1080 cells with Hsp60 antibody (Cat#61476, 1:1,000). Nuclei were stained blue with DAPI; Hsp60 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Hsp60 antibody (Cat#61476). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Hsp60 antibody (Cat#61476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Hsp60 antibody (Cat#61476). Hsp60 expression in wild type (WT) and Hsp60 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Hsp60 antibody (Cat#61476, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Hsp60 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Hsp60 antibody (Cat#61476, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Hsp60 antibody (Cat#61476, 1:1,000), Top panel: wild-type (WT); Bottom panal: Hsp60 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Hsp60 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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