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KD-Validated Anti-LDL receptor related protein 8 Rabbit Monoclonal Antibody #61482

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Flow cytometric analysis of LDL receptor related protein 8 expression in HepG2 cells using LDL receptor related protein 8 antibody (Cat#61482, 1:2,000). Green, isotype control; red, LDL receptor related protein 8.
Immunocytochemical staining of HepG2 cells with LDL receptor related protein 8 antibody (Cat#61482, 1:1,000). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-LDL receptor related protein 8 antibody (Cat#61482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-LDL receptor related protein 8 antibody (Cat#61482). LDL receptor related protein 8 expression in wild type (WT) and LDL receptor related protein 8 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of LDL receptor related protein 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-LDL receptor related protein 8 antibody (Cat#61482, 1:1,000), Top panel: wild-type (WT); Bottom panal: LDL receptor related protein 8 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    LRP8
  • 货号：
    61482
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-LDL receptor related protein 8 Rabbit Monoclonal Antibody
Aliases LRP8; LDL Receptor Related Protein 8; APOER2; LRP-8; HSZ75190; MCI1; Low Density Lipoprotein Receptor-Related Protein 8, Apolipoprotein E Receptor; Low-Density Lipoprotein Receptor-Related Protein 8; Apolipoprotein E Receptor 2; ApoE Receptor 2
Background

Gene Name: LRP8

NCBI Gene Entry: 7804

UniProt Entry: Q14114
Application Information

Molecular Weight: Predicted, 106 kDa, observed, 106 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2520

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ApoER2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of LDL receptor related protein 8 expression in HepG2 cells using LDL receptor related protein 8 antibody (Cat#61482, 1:2,000). Green, isotype control; red, LDL receptor related protein 8.
  • Immunocytochemical staining of HepG2 cells with LDL receptor related protein 8 antibody (Cat#61482, 1:1,000). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-LDL receptor related protein 8 antibody (Cat#61482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-LDL receptor related protein 8 antibody (Cat#61482). LDL receptor related protein 8 expression in wild type (WT) and LDL receptor related protein 8 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of LDL receptor related protein 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of Hela cells using anti-LDL receptor related protein 8 antibody (Cat#61482, 1:1,000), Top panel: wild-type (WT); Bottom panal: LDL receptor related protein 8 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    LRP8
  • 货号：
    61482
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of LDL receptor related protein 8 expression in HepG2 cells using LDL receptor related protein 8 antibody (Cat#61482, 1:2,000). Green, isotype control; red, LDL receptor related protein 8.
Immunocytochemical staining of HepG2 cells with LDL receptor related protein 8 antibody (Cat#61482, 1:1,000). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-LDL receptor related protein 8 antibody (Cat#61482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-LDL receptor related protein 8 antibody (Cat#61482). LDL receptor related protein 8 expression in wild type (WT) and LDL receptor related protein 8 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of LDL receptor related protein 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-LDL receptor related protein 8 antibody (Cat#61482, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-LDL receptor related protein 8 antibody (Cat#61482, 1:1,000), Top panel: wild-type (WT); Bottom panal: LDL receptor related protein 8 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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