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KD-Validated Anti-Methionyl aminopeptidase 2 Rabbit Monoclonal Antibody #61489

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Flow cytometric analysis of Methionyl aminopeptidase 2 expression in HepG2 cells using Methionyl aminopeptidase 2 antibody (Cat#61489, 1:2,000). Green, isotype control; red, Methionyl aminopeptidase 2.
Immunocytochemical staining of HepG2 cells with Methionyl aminopeptidase 2 antibody (Cat#61489, 1:1,000). Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Methionyl aminopeptidase 2 antibody (Cat#61489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Methionyl aminopeptidase 2 antibody (Cat#61489). Methionyl aminopeptidase 2 expression in wild type (WT) and methionyl aminopeptidase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:5000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Methionyl aminopeptidase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:1,000), Top panel: wild-type (WT); Bottom panal: Methionyl aminopeptidase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    METAP2
  • 货号：
    61489
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Methionyl aminopeptidase 2 Rabbit Monoclonal Antibody
Aliases METAP2; Methionyl Aminopeptidase 2; MNPEP; MAP2; P67; Initiation Factor 2-Associated 67 KDa Glycoprotein; Methionine Aminopeptidase 2; Peptidase M; P67eIF2; Testicular Tissue Protein Li 17; EIF-2-Associated P67 Homolog; Peptidase M 2; EC 3.4.11.18; P67EIF2; MetAP 2; MAP 2
Background

Gene Name: METAP2

NCBI Gene Entry: 10988

UniProt Entry: P50579
Application Information

Molecular Weight: Predicted, 53 kDa, observed, 67 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2565

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Methionine Aminopeptidase 2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Methionyl aminopeptidase 2 expression in HepG2 cells using Methionyl aminopeptidase 2 antibody (Cat#61489, 1:2,000). Green, isotype control; red, Methionyl aminopeptidase 2.
  • Immunocytochemical staining of HepG2 cells with Methionyl aminopeptidase 2 antibody (Cat#61489, 1:1,000). Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Methionyl aminopeptidase 2 antibody (Cat#61489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Methionyl aminopeptidase 2 antibody (Cat#61489). Methionyl aminopeptidase 2 expression in wild type (WT) and methionyl aminopeptidase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:5000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Methionyl aminopeptidase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:2,000) and analyzed using CytoFLEX.
  • Immunocytochemical staining of HeLa cells using anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:1,000), Top panel: wild-type (WT); Bottom panal: Methionyl aminopeptidase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    METAP2
  • 货号：
    61489
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Methionyl aminopeptidase 2 expression in HepG2 cells using Methionyl aminopeptidase 2 antibody (Cat#61489, 1:2,000). Green, isotype control; red, Methionyl aminopeptidase 2.
Immunocytochemical staining of HepG2 cells with Methionyl aminopeptidase 2 antibody (Cat#61489, 1:1,000). Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Methionyl aminopeptidase 2 antibody (Cat#61489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Methionyl aminopeptidase 2 antibody (Cat#61489). Methionyl aminopeptidase 2 expression in wild type (WT) and methionyl aminopeptidase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:5000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Methionyl aminopeptidase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-Methionyl aminopeptidase 2 antibody (Cat#61489, 1:1,000), Top panel: wild-type (WT); Bottom panal: Methionyl aminopeptidase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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